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human cdx2 cdna  (Bio-Rad)


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    Structured Review

    Bio-Rad human cdx2 cdna
    Human Cdx2 Cdna, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 2482 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+cdx2+cdna/Filter/10__1074_slash_jbc__m307435200-118-37-52
    Average 96 stars, based on 2482 article reviews
    human cdx2 cdna - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Silencing of CDX2 Expression in Colon Cancer via a Dominant Repression Pathway
    Article Snippet: The 32P-labeled RNA solution was hybridized to DNA immobilized on strips of nitrocellulose filters (BioRad). .. Five micrograms of linearized and denatured DNA plasmids such as pBS vector without insert as a negative control, pBS vector containing human GAPDH cDNA as a positive control to normalize the hybridization signals, and pBS vector containing human CDX2 cDNA (codon 2–164) were spotted on the filter using a DNA slot apparatus (Bio-Rad). .. Hybridization was carried out at 65 °C for 36 h, and the filter was washed in 2 SSC (1 SSC is 150 mM NaCl and 15 mM sodium citrate, pH 7.0) at 65 °C for 1 h twice, incubated with 2 SSC containing 10 g/ml RNase A at 37 °C for 30 min, and then washed again in 2 SSC at 37 °C for 1 h. The filter was dried and exposed to BioMax-MS film for 5–7 days.

    Negative Control:

    Article Title: Silencing of CDX2 Expression in Colon Cancer via a Dominant Repression Pathway
    Article Snippet: The 32P-labeled RNA solution was hybridized to DNA immobilized on strips of nitrocellulose filters (BioRad). .. Five micrograms of linearized and denatured DNA plasmids such as pBS vector without insert as a negative control, pBS vector containing human GAPDH cDNA as a positive control to normalize the hybridization signals, and pBS vector containing human CDX2 cDNA (codon 2–164) were spotted on the filter using a DNA slot apparatus (Bio-Rad). .. Hybridization was carried out at 65 °C for 36 h, and the filter was washed in 2 SSC (1 SSC is 150 mM NaCl and 15 mM sodium citrate, pH 7.0) at 65 °C for 1 h twice, incubated with 2 SSC containing 10 g/ml RNase A at 37 °C for 30 min, and then washed again in 2 SSC at 37 °C for 1 h. The filter was dried and exposed to BioMax-MS film for 5–7 days.

    Positive Control:

    Article Title: Silencing of CDX2 Expression in Colon Cancer via a Dominant Repression Pathway
    Article Snippet: The 32P-labeled RNA solution was hybridized to DNA immobilized on strips of nitrocellulose filters (BioRad). .. Five micrograms of linearized and denatured DNA plasmids such as pBS vector without insert as a negative control, pBS vector containing human GAPDH cDNA as a positive control to normalize the hybridization signals, and pBS vector containing human CDX2 cDNA (codon 2–164) were spotted on the filter using a DNA slot apparatus (Bio-Rad). .. Hybridization was carried out at 65 °C for 36 h, and the filter was washed in 2 SSC (1 SSC is 150 mM NaCl and 15 mM sodium citrate, pH 7.0) at 65 °C for 1 h twice, incubated with 2 SSC containing 10 g/ml RNase A at 37 °C for 30 min, and then washed again in 2 SSC at 37 °C for 1 h. The filter was dried and exposed to BioMax-MS film for 5–7 days.

    Hybridization:

    Article Title: Silencing of CDX2 Expression in Colon Cancer via a Dominant Repression Pathway
    Article Snippet: The 32P-labeled RNA solution was hybridized to DNA immobilized on strips of nitrocellulose filters (BioRad). .. Five micrograms of linearized and denatured DNA plasmids such as pBS vector without insert as a negative control, pBS vector containing human GAPDH cDNA as a positive control to normalize the hybridization signals, and pBS vector containing human CDX2 cDNA (codon 2–164) were spotted on the filter using a DNA slot apparatus (Bio-Rad). .. Hybridization was carried out at 65 °C for 36 h, and the filter was washed in 2 SSC (1 SSC is 150 mM NaCl and 15 mM sodium citrate, pH 7.0) at 65 °C for 1 h twice, incubated with 2 SSC containing 10 g/ml RNase A at 37 °C for 30 min, and then washed again in 2 SSC at 37 °C for 1 h. The filter was dried and exposed to BioMax-MS film for 5–7 days.



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    <t>CDX2</t> and ISC marker expression in 28 fresh frozen-non-tumorous gastric tissues by quantitative real-time PCR (qRT-PCR) analysis. (A) Gastric mucosae show a wide range of CDX2 expression levels, representing the various degrees of intestinal metaplasia. OLFM4 ( p < 0.0001, r 2 = 0.56) (B) and EPHB2 ( p < 0.0001, r 2 = 0.52) (C) expression increases significantly along with CDX2 levels, whereas BMI1 ( p = 0.0002, r 2 = 0.42) (D) decreases with CDX2 expression.
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    <t>CDX2</t> and ISC marker expression in 28 fresh frozen-non-tumorous gastric tissues by quantitative real-time PCR (qRT-PCR) analysis. (A) Gastric mucosae show a wide range of CDX2 expression levels, representing the various degrees of intestinal metaplasia. OLFM4 ( p < 0.0001, r 2 = 0.56) (B) and EPHB2 ( p < 0.0001, r 2 = 0.52) (C) expression increases significantly along with CDX2 levels, whereas BMI1 ( p = 0.0002, r 2 = 0.42) (D) decreases with CDX2 expression.
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    Image Search Results


    CDX2 and ISC marker expression in 28 fresh frozen-non-tumorous gastric tissues by quantitative real-time PCR (qRT-PCR) analysis. (A) Gastric mucosae show a wide range of CDX2 expression levels, representing the various degrees of intestinal metaplasia. OLFM4 ( p < 0.0001, r 2 = 0.56) (B) and EPHB2 ( p < 0.0001, r 2 = 0.52) (C) expression increases significantly along with CDX2 levels, whereas BMI1 ( p = 0.0002, r 2 = 0.42) (D) decreases with CDX2 expression.

    Journal: PLoS ONE

    Article Title: Intestinal Stem Cell Markers in the Intestinal Metaplasia of Stomach and Barrett’s Esophagus

    doi: 10.1371/journal.pone.0127300

    Figure Lengend Snippet: CDX2 and ISC marker expression in 28 fresh frozen-non-tumorous gastric tissues by quantitative real-time PCR (qRT-PCR) analysis. (A) Gastric mucosae show a wide range of CDX2 expression levels, representing the various degrees of intestinal metaplasia. OLFM4 ( p < 0.0001, r 2 = 0.56) (B) and EPHB2 ( p < 0.0001, r 2 = 0.52) (C) expression increases significantly along with CDX2 levels, whereas BMI1 ( p = 0.0002, r 2 = 0.42) (D) decreases with CDX2 expression.

    Article Snippet: CDX2 cDNA (pCMV6-CDX2) was purchased from OriGene (Rockville, MD, USA).

    Techniques: Marker, Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR

    ISC expression in histologically distinct gastric lesions (n = 20), including normal antrum without intestinal metaplasia (IM) (n = 4), chronic active gastritis without IM (n = 3), gastro-intestinal mixed (GI) type IM (n = 6), solely intestinal (I) type IM (n = 5), and small intestine (n = 2). Hematoxylin and eosin staining of each gastric lesion (Magnification ×100, A). Claudin 18 expression gradually decreases ( p < 0.0001, B), while CDX2 ( p < 0.0001, C) increases with intestinal metaplastic progression. Among ISC markers, OLFM4 ( p = 0.008, D) and EPHB2 ( p = 0.001, E) expression gradually increases with metaplasia while BMI1 ( p = 0.002) (F) and LRIG1 ( p = 0.0006, G) expression is reduced.

    Journal: PLoS ONE

    Article Title: Intestinal Stem Cell Markers in the Intestinal Metaplasia of Stomach and Barrett’s Esophagus

    doi: 10.1371/journal.pone.0127300

    Figure Lengend Snippet: ISC expression in histologically distinct gastric lesions (n = 20), including normal antrum without intestinal metaplasia (IM) (n = 4), chronic active gastritis without IM (n = 3), gastro-intestinal mixed (GI) type IM (n = 6), solely intestinal (I) type IM (n = 5), and small intestine (n = 2). Hematoxylin and eosin staining of each gastric lesion (Magnification ×100, A). Claudin 18 expression gradually decreases ( p < 0.0001, B), while CDX2 ( p < 0.0001, C) increases with intestinal metaplastic progression. Among ISC markers, OLFM4 ( p = 0.008, D) and EPHB2 ( p = 0.001, E) expression gradually increases with metaplasia while BMI1 ( p = 0.002) (F) and LRIG1 ( p = 0.0006, G) expression is reduced.

    Article Snippet: CDX2 cDNA (pCMV6-CDX2) was purchased from OriGene (Rockville, MD, USA).

    Techniques: Expressing, Staining

    Both BE and adenocarcinoma sections from patients with adenocarcinoma of gastroesophageal junction (A) (n = 2) express higher levels of CDX2 (***, p < 0.005) (B) and intestinal stem cell (ISC) markers including OLFM4 (**, p < 0.01) (C), EPHB2 (*, p < 0.05) (D), and PROM1 (*, p < 0.05) (E) compared to normal esophageal mucosa. RNA in situ hybridization demonstrates that LGR5 expression in basal metaplastic glands (G) colocalizes with ASCL2 (H) and OLFM4 (I). (A) and (F) show representative sections stained with hematoxylin and eosin. Arrowheads refer to intestinal-like stem cells that express all three markers. Magnification: A, ×1.25; F ×40; G-I ×400. Eso, esophagus; BE, Barrett’s esophagus; EC, esophageal cancer.

    Journal: PLoS ONE

    Article Title: Intestinal Stem Cell Markers in the Intestinal Metaplasia of Stomach and Barrett’s Esophagus

    doi: 10.1371/journal.pone.0127300

    Figure Lengend Snippet: Both BE and adenocarcinoma sections from patients with adenocarcinoma of gastroesophageal junction (A) (n = 2) express higher levels of CDX2 (***, p < 0.005) (B) and intestinal stem cell (ISC) markers including OLFM4 (**, p < 0.01) (C), EPHB2 (*, p < 0.05) (D), and PROM1 (*, p < 0.05) (E) compared to normal esophageal mucosa. RNA in situ hybridization demonstrates that LGR5 expression in basal metaplastic glands (G) colocalizes with ASCL2 (H) and OLFM4 (I). (A) and (F) show representative sections stained with hematoxylin and eosin. Arrowheads refer to intestinal-like stem cells that express all three markers. Magnification: A, ×1.25; F ×40; G-I ×400. Eso, esophagus; BE, Barrett’s esophagus; EC, esophageal cancer.

    Article Snippet: CDX2 cDNA (pCMV6-CDX2) was purchased from OriGene (Rockville, MD, USA).

    Techniques: RNA In Situ Hybridization, Expressing, Staining